Jeg-3 cells
Web13 mar 2024 · JEG-3 cells were cultured in 12-well plates (200 cells per well) overnight, then treated with various concentrations of α-solanine (10, 20 and 30 μM) or vehicle for 24 h. After treatment, cells were cultured in complete medium for 7 … Web23 set 2024 · The JEG-3 or BeWo cells in the logarithmic phase (8,000 cells/well) were trypsinized and added into 96-well plates for a 24-h pre-incubation at 37 ° C. Following incubation for 4 h at 37 ° C, CCK8 solution (10 µ l) was injected to each well.
Jeg-3 cells
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Web1 mag 2002 · JEG-3 cells (5×10 5 cells in 30 mm dishes) incubated overnight with retinol (with or without ethanol or 4MP) and the conditioned media were placed on the F9-1.8 … Web1 gen 2024 · JEG-3 was further evolved to AC1-1-cells, as a hypoxanthine-guanine phosphoribosyltransferase-negative mutant [10]. AC1-1 cells were transformed into two separate cell lines by somatic hybridization: first trimester trophoblast ACH-3P cells [11] and, in 1997, to primary male third trimester trophoblast cells ACH-1P cells [12].
Web24 ago 2015 · Cell culture. Human placental choriocarcinoma JEG-3 cells (ATCC) were cultured at 37°C in 5 % CO 2 in growth medium consisting of MEM Alpha Modification medium (Hyclone) supplemented with 10 % (v/v) fetal bovine serum (FBS) (Hyclone), 100 μg/ml streptomycin (Hyclone), 100 U/ml penicillin (Hyclone) and 100 μg/ml non-essential … Web24 ott 2024 · Based on the experimental design, JEG-3 cells were seeded in 6 well plates, 12-well plates, or 96-well plates at 5 × 10 5 cells per well, 2.5 × 10 5 cells per well, or 15 …
Web1 set 2024 · Co-culture of human decidual or peripheral NK cells with a trophoblast-like cell line JEG-3 infected with L. monocytogenes significantly reduced intracellular bacteria levels but did not result in ... Web26 gen 2024 · JEG-3 cells were cultured in MEM containing 10% fetal bovine serum, and plasmocin (0.01%). All the cells used the present study were obtained from ATCC and periodically tested for mycoplasma.
WebHTR-8/SVneo or JEG-3 cells (5 × 10 3 cells/well) were seeded into sterile plates of 96 wells in complete medium (DMEM/F12, MEM, 10% FBS). Cells were incubated and cultured with TCS of different concentrations for 24 h.
WebHTR-8/SVneo and JEG-3 cells were transfected with siRNA-PTPRO-1/2 or siRNA-NC. (a) RT-qPCR was employed to detect the mRNA level of PTPRO in HTR-8/ SVneo cells. concept art internship melbWeb1 mag 2016 · While JEG-3 cells secreted high amounts of hCG, only low hCG levels were detectable in the supernatant of HTR-8 cells (E). Moreover, no hCG was present in the … concept art darkstalkers feliciaWeb18 set 2024 · Hence, JEG-3 line cells in the presence of placenta secreted factors at preeclampsia reduced the sensitivity to leptin, cytokines IFNγ, TNFα, LIF, chemokines IP … concept artist internWebJeg-3 choriocarcinoma cells express class-I MHC HLA-G and low levels of a novel HLA-C product. The functional significance of such novel MHC class-I expression in regard of … concept art hotel roomWebJEG-3 cells The human choriocarcinoma-derived JEG-3 cells used in these experiments were obtained from the American Type Culture Collection (Rockville, MD) [17,18]. The cells were cultured in DMEM supplemented with 10% FBS and passaged by brief enzymatic digestion using 0.1% trypsin-EDTA. Invasion and growth of L. monocytogenes in JEG-3 … eco products ep s005WebTo give you confidence in the health of your cells every step of the way, we’ve highlighted the technologies and products within cell biology that are critical to maintaining optimal … eco products ep-scs5Web9 mag 2024 · The miR-371-3 cluster (including miR-371, -372, and -373) is expressed in primary trophoblast cells, JEG-3 cells, and their hybrids, but not in immortalized HTR-8/SVneo Mice have homologous cluster miR-290-296 to the human miR-371-3 cluster . miRNAs of miR371-3 cluster region act as oncogenes or tumor suppressor when up or … eco products ep-s012-w