Phis2自激活

Author: xvaw

August undefined, 2024

Webbactivation domain in pGADT7-Rec2. The target DNA sequence, or Bait Sequence, is cloned into pHIS2.1 as one copy or tandem repeats. Interaction between a DNA-binding protein … Webb1 mars 2024 · C. Yeast one-hybrid assay detect the specific binding ability of VvWRKY30 to W-BOX. pGADT7-empty and pHIS2.1-W-BOX, AD- VvWRKY30 and pHIS2.1-empty were …

酵母杂交实验常见问题——酵母转化篇_菌落_平板_效率

http://www.qiyuebio.com/product/61298 Webb上海懋康生物科技有限公司是一家涉足于生命科学和生物技术领域研究的试剂、仪器和实验室消耗品与实验服务工作，主要从事细胞生物学、植物学、分子生物学、免疫学、生物化学、蛋白组学。生物制药与诊断试剂研发生产等领域。本公司秉承“以人为本，以诚为信、合同守信”的经营理念。 data protection act outline

Nature子刊：CAR-T细胞疗法目前的局限性和潜在策略 - 癌症研究 …

http://html.rhhz.net/SWJSTB/html/2024-12-110.htm Webb5 nov. 2015 · 酵母双杂交 AH109,Y187. 下面所有质粒和菌株，均为本公司实验室亲自验证过，可以提供，载体来源于 promega,addgene,clontech,novagen,invitrogen 可提供质粒图谱，可提供近万种质粒载体、菌株、基因和细胞株, 并可提供实验技术外包服务, 如基因克隆、质粒构建、蛋白原核 ... http://www.bioon.com.cn/doc/showarticle.asp?newsid=96627 data protection act of ghana

上海宝吉生物-酵母文库构建，酵母文库筛选，酵母双杂交文库构建 …

WebbpHIS2 PVT4037 2ug Lot no 1901093 Store at -20℃ Expiry date June.2024 pHIS2 Information Promoters: TRP1, T7, T3 Replicon: pUC ori, FI ori Plasmid classification: … WebbpHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker™ One-Hybrid Library Construction & Screening Kit (#K1617-1). It contains a HIS3 nutritional reporter gene,located downstream of a multiple cloning site ... bits hostel fee structureWebbPhis2 1 Bait Plasmid, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Home > Search Results > TaKaRa > phis2. plasmid phis2 1 TaKaRa is a verified supplier TaKaRa manufactures this product . About; News ... data protection act most recent

"Webb本文针对酵母单杂技术的基本原理、基础知识、主要用途做了简要介绍；并以Y1Hgold（pAbAi-bait）和Y187（pHis2-bait）为例，介绍了自激活检测的目的以及主要 … " - Phis2自激活

Phis2自激活

Webb1 mars 2024 · MdGSTF6-p-pHIS2 vectors were first grown on −Trp/−His medium containing 3-amino-1,2,4-triazole (3-AT) for screening. The concentration of 3-AT used was 100 mM. Webb中国微生物菌种查询网专业提供pHIS2查询与购买，编号为bio-109465,微生物菌种查询网(The Website of Microorganism for Search)是集菌种信息、细胞信息、质粒信息及培养基信息于一体的微生物相关查询网站,并提供美国ATCC、日本JCM等进口品牌的菌种购买服务,数据庞大，内容丰富，欢迎您的查阅！

Did you know?

Webb2024年4月17日讯/ 生物谷 BIOON/---嵌合抗原受体（CAR）T细胞（CAR-T）疗法是革命性的，因为它已经产生了非常有效和持久的临床反应。. CAR是一种工程化 ... Webb14 apr. 2024 · 目的：筛选与启动子pHIS2-A互作的蛋白。方法：以启动子A构建到酵母单杂交启动子载体pHIS2，文库质粒为pGADT7-中山杉文库。材料： 1．启动子克隆：pHIS2-A。根据实验需求进行扩增并酶切构建载体，两端的EcoRI和. SacI酶切位点，序列如下：

http://muchong.com/t-9775583-1 http://www.biofeng.com/zaiti/jiaomu/pHis2.html

http://www.zoonbio.com/molecular/yeast-one-hybrid-procedure.html Webb1 dec. 2024 · AD/pATM1-pHIS2 and AD-ATM1/pATM1-pHIS2 were cotransformed into the Y187 strain and cultured on SD/-Trp/-Leu for 2–3 days at 30 °C. Then, healthy and consistent clones were diluted, ...

Webb16 mars 2024 · 2. 制备感受态并将pHis2-DNA转化进入感受态细胞； 3. SD/-Trp平板检测转化结果。二、自激活检测. 1. 制备不同3-AT浓度梯度的SD/-His/-Trp平 …

Webb载体描述. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker™ One- Hybrid Library Construction & Screening Kit. It contains a HIS3 nutritional reporter gene, located downstream of a multiple cloning ... bit shut.comWebb使用phis2载体，将启动子序列构建在his3报告基因前面的多克隆位点上，对应的转录因子构建在ad载体上即可。使用y187菌株，它相对于ah109菌株在基因组上少了his的报告基 … data protection act legislation.govWebb挑取pHIS2的单个克隆接种于含有Kan（50 mg/L）的LB液体培养基中于37℃摇床中180 r/min过夜培养，用质粒小量提取试剂盒提取质粒后用Eco R Ⅰ和Sac Ⅰ进行双酶切，并纯化回收。 1.2.3 连接及转化. 将稀释后的复性双链DNA片段与切后的pHIS2进行连接，反应体系如 … data protection act pakistanhttp://www.biogenesci.com/ bitshute.com kerry cassidyWebbClose You have no items in your shopping cart.. Home; Plasmid; p53- His2 Model: PVT11251 data protection act or gdprWebbpHIS2 Vector Information PT3705-5 Sold as part of Catalog #K1617-1 Restriction Map and Multiple Cloning Site (MCS) of pHIS2 Vector. Unique restriction sites are in bold. *Note: The Sac II site is resistant to cleavage. Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding ... bitshutdownWebb27 mars 2024 · 酵母单杂交.ppt,酵母单杂交系统吉林大学植物科学学院 2010级硕士酵母单杂交系统原理质粒和菌株质粒：诱饵质粒pHIS2，文库质粒为pGATD7-rec2 诱饵质粒上连接有目标靶序列，最小启动子和报告基因文库质粒则和反转录得到的真核生物cDNA连接，可以表达AD杂合蛋白酵母菌株：Y187 GAL4基因是缺失型的 ... data protection act penalty