Taq dna polymerase 5 u/μl

Author: yjcu

August undefined, 2024

WebReverse Primer， 10 μM 2 μl 0.4 μM Template DNA <0.5 μg <0.5 μg/50 μl Taq DNA Polymerase， 5 U/μl ddH 2 O up to 50 μl 注意： 1）一般实验中退火温度比扩增引物的熔解温度Tm低5℃，无法得到理想的扩增效率时，适当降 Web製品説明 TaKaRa Taq は、 Thermus aquaticus DNA Polymerase遺伝子をクローニングして改変したものを大腸菌にて大量に発現させ、高度に精製した分子量94 kDaの耐熱性DNAポリメラーゼである。天然の Taq DNA Polymeraseと同じ機能を有する。本製品で使用している TaKaRa Taq HSは、抗 Taq 抗体と TaKaRa Taq を混合したもので、 …

ROCHE FastStart™ Taq DNA Polymerase, 5 U/µl - Thomas Sci

WebAccurate Taq enzyme 是一种性能优越的热稳定性 DNA 聚合酶，是将Thermus aquaticus DNA Polymerase gene 克隆表达得到。使用本品得到的 PCR 产物 3’ 端带有一个 A 碱 … WebTaq DNA Polymerase is a highly processive 5′→3′ DNA polymerase that lacks 3′→5′ exonuclease activity. It is a single polypeptide chain with a molecular weight of … state elevations united states

ROCHE Taq DNA Polymerase 5 U/m 11146165001 MERCK …

WebProperties. AptaTaq exo DNA Polymerase is active at temperature above +60 to +65°C and inactive below +55°C. This hot start feature eliminates the risk of nonspecific primer … WebTaq DNA Polymerase is the recombinant full-length version of the thermostable enzyme from the eubacterium Thermus aquaticus BM, expressed in E. coli. Enzyme acivities: … WebTaq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The enzyme consists of a single polypeptide with a molecular weight of 94 … state elimination method

iTaq™ DNA Polymerase, 250 U (5 U/µl), 50 µl #1708870 Bio-Rad

WebThermo Scientific DreamTaq DNA Polymerase is an enhanced Taq DNA polymerase optimized for all standard PCR applications. It ensures higher sensitivity, longer PCR … WebPwo DNA Polymerase is a highly processive thermostable 5′-3′ DNA polymerase and possesses a 3′–5′ exonuclease activity, ... compared to Taq DNA Polymerase′s half-life of less than 5 minutes at this ... dCTP are incubated with 0.01 to 0.1 U Pwo DNA Polymerase in 50 μl incubation buffer with a paraffin oil overlay at +70 °C for 30 ... state eligibility requirements for medicaidWeb3 apr 2011 · Taq DNA polymerase is an 832-amino acid protein with an inferred molecular weight of 93,920 and a specific activity of 292,000 units/ mg; optimal polymerization activity is achieved at 75–80 ° C, with half-maximal activity at … state eligibility test 2022 maharashtra

"WebVolume activity: ≥5 U/μL Unit definition: One unit Taq DNA Polymerase is defined as the amount of heat-activated enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75°C under standard assay conditions. " - Taq dna polymerase 5 u/μl

Taq dna polymerase 5 u/μl

WebTaq DNA Polymerase, 5 U/μl optimum pH ~9.0 (20 °C), optimum reaction temp. 72 °C; Synonyms: dna amplification,pcr,polymerase, pcr, primer extension, dna … WebThermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5'→3' synthesis of …

Did you know?

WebBst Max DNA Polymerase recombinant, expressed in E. coli, Salt and Inhibitor Tolerant; Synonyms: Bst DNA polymerase,Isothermal DNA Polymerase; ... FastStart ™ Taq DNA Polymerase, 5 U/μl. dNTPs included: no, hotstart, suitable for PCR. View Price and Availability. Sigma-Aldrich. PIR00. WebWe generally recommend using Taq DNA Polymerase at a concentration of 25 units/ml (1.25 units/50 μl reaction). However, the optimal concentration of Taq DNA Polymerase …

WebThe kit contains KAPA3G DNA Polymerase, a novel third-generation (3G) enzyme that was engineered via our directed evolution technology for improved tolerance to common plant … WebTaq DNA Polymerase is used in a variety of techniques: • The enzyme activity is stable during prolonged incubation at high temperatures (+95°C) and can therefore be used to …

WebTaKaRa Ex Taq (5 U/μl) 0.25 μl 10X Ex Taq Buffer (Mg2＋ plus) (20 mM) 5 μl dNTP Mixture (2.5 mM each) 4 μl Template < 500 ng Primer 1 0.2 - 1.0 μM (final conc.) Primer 2 0.2 - 1.0 μM (final conc.) Sterile purified water up to 50 μl Example of PCR conditions : When amplifying a 1 kb DNA fragment 98℃ 10 sec 55℃ 30 sec 72℃0.5% 1 min Web反应体系（50 μl）组分名称反应终浓度加入量 Accurate Taq DNA Polymerase （5U/μl） 1.25 U 0.25 μl 10X Taq PCR Buffer (Mg2+ plus) 1 x 5 μl dNTP Mix (10mM each) 0.2 mM 1 μl Template < 500 ng - Primer F 0.2 – 1.0 μM - Primer R 0.2 – 1.0 μM - RNase free water — Up to 50 μl 实验操作 Accurate Biotechnology(Hunan ...

WebFastStart Taq DNA Polymerase is designed for hot start PCR and has to be heat-activated in the beginning of the reaction protocol. Enzyme acivities: Highly processive 5'-3' DNA …

WebPlatinum™ Taq DNA Polymerase, High Fidelity, is ideal for amplification of DNA fragments when high yields and robust amplification are required. High fidelity is provided by a … state eligibility test 2022 applyWebThermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The … state eligibility test madhya pradeshWebSource: Recombinant Thermus aquaticus DNA polymerase expressed in E. coli Concentration: 5.0 U/μl Purity: 95% homogeneous by SDS-PAGE Endonuclease: None detected Amplification effiency: Functional PCR Storage: –20°C. ... •100 U, 500 U, or 5 × 500 UNovaTaq DNA Polymerase •1 or 2 or 7 × 1.5 ml10X NovaTaq Buffer with MgCl ... state emblem of chhattisgarhWeb24 nov 2024 · 1.5 U Taq DNA polymerase 5 μL DNA template: 1 cycle: 94 °C for 10 min 30 cycles: 94 °C for 60 s 60 °C for 60 s 72 °C for 60 s 1 cycle: 72 °C for 5 min: papGI: F: TCGTGCTGAGGTCCGGAATTT R: TGGCATCCCCCAACATTATCG: 461: 5 μL PCR buffer 10 × 2 mM MgCl 2 200 μM dNTP 0.5 μM of each primers F & R 1.5 U Taq DNA … state eligibility test west bengalWeb250 U (5 U/µl), hot-start polymerase for PCR and real-time PCR, includes 1.25 ml each 10x PCR buffer and 50 mM MgCl2. Skip to main content My Account. Log In/Register; My … state emblem of manipurWebSupreme NZYTaq II DNA polymerase is an engineered version of NZYTaq II DNA polymerase displaying a hot-start-like PCR capacity. The enzyme activity at room temperature is limited, avoiding extension of non-specifically annealed primers or primer-dimers and thus providing higher specificity, sensitivity and yield during DNA amplification. state emblem of israel nytWebDescription. Storage: −20°C. UNSPSC Code: 12352200. Components: FastStart Taq DNA Polymerase, in storage and dilution buffer 5 U/μl; PCR Reaction Buffer, with 20 mM MgCl 2 10x concentrated; PCR Reaction Buffer, without MgCl 2 10x concentrated; MgCl 2 Stock Solution 25 mM; GC-RICH Solution 5x concentrated. RIDADR: NONH for all modes of ... state emblem of andhra pradesh