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WebMembranes were then washed in TTBS and incubated with 1:10,000 HRP-conjugated anti-rabbit IgG (GE Healthcare Canada at room temperature for 1 h). Membranes were washed and reprobed with an antibody raised against β-actin (ACTB) as a loading control, whereas blots for ATF6 (50 kDa) were stripped to be re-incubated with ACTB as the proteins are of …
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